Modeling Diverse Clots that Cause Stroke

Clots that cause stroke can come from numerous sources in the body, and they differ in composition, age, and morphology. NTI has developed methods to create a wide range of clot analogs that reflect clinical clots, in order to better understand these challenges in treating ischemic stroke.
 

Fresh thrombus encapsulating mature fibrin core
 

Young erythrocyte-rich clot analogue
 

Partially matured clot analogue

 

Heterogeneous clot with erythrocyte-rich and fibrin rich regions

Layered clot with mature fibrin base and fresh erythrocyte deposition
 

Composite clot with calcific inclusion
 

Highly organized fibrin-rich clot analogue

Fresh, erythrocyte-rich clot analogue
 

Shear-induced fibrin-rich prosthetic valve clot analogue
 

Staining of four different clot types (x20 magnification). MSB stained fibrin red and erythrocytes yellow:

H&E (hematoxylin and eosin staining)
 

MSB (martius scarlet blue staining)​​
 

Clot replete with red blood cells (RBCs)
 

Thrombin induced clot predominately composed of RBCs with interspersed bands of fibrin
 

Dynamically formed clot, demonstrating a predominance of fibrin with collections of RBCs
 

Fibrin rich clot, showing delicate fine fibrin strands